Immunofluorescence Staining of Paraffin Sections Step by Step
نویسندگان
چکیده
منابع مشابه
Immunofluorescence staining of paraffin sections: creating DAB staining like virtual digital images using CMYK color conversion.
Crystal violet treatment of formalin fixed paraffin embedded tissue slides greatly reduces the endogenous autofluorescence, and allows immunofluorescence (IF) staining with FITC or Alexa488 conjugated antibodies. Using cold CCD camera to capture the fluorescence images makes this staining method very sensitive. Here we show that combination of IF with the simultaneous recording of crystal viole...
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ژورنال
عنوان ژورنال: Frontiers in Neuroanatomy
سال: 2020
ISSN: 1662-5129
DOI: 10.3389/fnana.2020.582218